Ted by the combined effect of tumor necrosis element (TNF)- plus be induced by (CHX) inside a TNF- dose-dependent manner. Additionally, the 4-related of could cycloheximide increased doses of IFN- (Figure 4B), indicating that the caspase activationER caspase four may be induced by increased doses of IFN- (Figure 4B), indicating that the caspase stress-induced apoptotic pathway, but not the extrinsic apoptotic pathway, may perhaps also contribute to 4-related ER stress-induced apoptotic cells. IFN–mediated cell apoptosis in HeLa pathway, but not the extrinsic apoptotic pathway, may perhaps also contribute to IFN–mediated cell apoptosis in HeLa cells.Int. J. Mol. Sci. 2016, 17, 1832 Int. J. Mol. Sci. 2016, 17,Int. J. Mol. Sci. 2016, 17,6 of 13 6 of6 ofFigure 4. IFN- mediated cell apoptosis in HeLa cells also associated with ER stress-induced Figure 4. IFN- mediated cell apoptosis in HeLa cells isis also related to ER stress-induced Figure 4. IFN- mediated cell apoptosis in HeLa cells is also connected with ER stress-induced apoptosis. (A) IFN- does not activate the extrinsic apoptotic pathway. The whole-cell lysates apoptosis. (A)IFN- does not activate the the extrinsic apoptotic pathway. The whole-cell lysates apoptosis. (A) from IFN- or not activate extrinsic apoptotic pathway. The whole-cell lysates cells IFN- does tumor necrosis aspect (TNF)-/cycloheximide(CHX)-treated HeLa prepared prepared from IFN- or IFN- or tumor necrosis issue (TNF)-/cycloheximide(CHX)-treated HeLa cells prepared from tumor necrosis factor (TNF)-/cycloheximide(CHX)-treated HeLa cells have been probed have been probed with anti-caspase ten, anti-caspase 8, anti-cleaved-caspase 8, anti-caspase 3, and with anti-caspase ten, anti-caspase 10,anti-cleaved-caspase eight, anti-caspase 3, and anti-cleaved-caspase were anti-cleaved-caspase 3 antibodies, and subjected to Western blot analysis. GAPDH anti-caspase 3, and probed with anti-caspase eight, anti-caspase eight, anti-cleaved-caspase 8, (glyceraldehyde3 antibodies, and subjected to Western blot to Western blot evaluation.35265-83-9 site GAPDH (glyceraldehydeanti-cleaved-caspase 3 antibodies, and subjected analysis.1427158-38-0 custom synthesis anGAPDH control; (B) IFN- activates 3-phosphate dehydrogenase) gene expression is served as internal (glyceraldehyde-3-phosphate dehydrogenase) gene expression reticulum (ER) stress-induced apoptosis in HeLa cells.PMID:23991096 The whole-cell is served as anis servedcontrol;internal manage; (B)caspase activates internal as an (B) IFN- activates IFN- 4-related caspase dehydrogenase) gene expression 3-phosphate4-related endoplasmic endoplasmic reticulum (ER) stress-induced apoptosis probed cells. The whole-cell lysates and lysates prepared from IFN–treated HeLa stress-induced apoptosis in HeLa cells. The whole-cell caspase 4-related endoplasmic reticulum (ER)cells werein HeLa with anti-caspase four antibodies ready fromanalyzed by Western blot. were probed with anti-caspase 4 antibodies and control. 4 antibodies and IFN–treated HeLa cells GAPDH HeLa cells had been probed an internal analyzed by Western lysates prepared from IFN–treated gene expression is served aswith anti-caspase The diagram blot. represents the quantitation with the relative caspase four under IFN- remedy in GAPDH gene expression is GAPDH gene expression protein expression represents the quantitation of analyzed by Western blot. served as an internal manage. The diagraminternal handle. The diagram is served as an HeLa cells the relativethe from four proteinof the relative caspase four treatment in HeLa statisti.
