Uantified having a Multiskan microplate spectrophotometer (Thermo LabSystems, Milford, MA).The
Uantified with a Multiskan microplate spectrophotometer (Thermo LabSystems, Milford, MA).The lysates were obtained by lysing cells in lysis buffer containing 50 mM Tris, pH7.four, 150 mM NaCl, 0.25 sodium deoxycholate,…