Rewarmed RPMI 1640 full medium containing 0.3 mM dibutyryl cAMP, 10 FBS, ten mM HEPES
Rewarmed RPMI 1640 complete medium containing 0.three mM dibutyryl cAMP, ten FBS, ten mM HEPES, 1 mM Na + pyruvate and maintained at 37 within a five CO2 humidified atmosphere.…