Nus the bone (lighter gray) equals the callus (darkest gray). The horizontal lines indicate where the masks had been cropped. (C) Mechanical testing setup for fractured bone and callus (darker gray) and intact bone (lighter gray). (TIF) Figure Sp-Akt IHC of fracture calluses at day 14 PF. (A) 106magnification of wild-type callus; (B) 206magnification of box from (A); (C) 106 magnification of Pten mutant callus; (D) 206 magnification of box from (C). p-Akt was expressed at a related level in each and every case. (TIF) p-Akt IHC of fracture calluses at day 21 PF. (A) 106magnification of wild-type callus; (B) 206magnification of box from (A); (C) 106 magnification of Pten mutant callus; (D) 206 magnification of box from (C).199105-03-8 Formula p-Akt was expressed at a similar level in each and every case. (TIF)Figure SFigure SQuantitation of western blots. (A) Mutant animals trended to possess more normalized p-Akt than wildtype animals. (B) Mutant animals trended to possess significantly less normalized Pten than wildtype animals. (TIF)p-S6 IHC of fracture calluses at day 14 PF(A) 106 magnification of wild-type callus; (B) 206 magnification of box from (A); (C) 106 magnification of Pten mutant callus; (D) 206 magnification of box from (C). pS6 was expressed at a equivalent level in each case. (TIF)Figure S3 H E of fracture calluses at day 7 PF. (A)magnification of wild-type callus; (B) 106magnification with the box from (A); (C) 46magnification of Pten mutant callus; and (D) 106 magnification with the box from (C). The fracture callus consisted of mainly fibroblast cells and chondrocytes in each and every case. (TIF)Figure SFigure S13 p-S6 IHC of fracture calluses at day 21 PF. (A) 106magnification of wild-type callus; (B) 206magnification of box from (A); (C) 106 magnification of Pten mutant callus; (D) 206magnification of box from (C). p-S6 was expressed at a comparable level in every case. (TIF) Figure S14 TRAP stain of fracture calluses at day 21 PF. (A) 106magnification of wild-type callus; (B) 206magnification of box from (A); (C) 106 magnification of Pten mutant callus; (D) 206 magnification of box from (C).4-(Methoxycarbonyl)nicotinic acid web TRAP staining was extra intense inside the mutant group. (TIF)H E of fracture calluses at day 21 PF. (A) 46 magnification of wild-type callus; (B) 106 magnification of box from (A); (C) 46magnification of Pten mutant callus; and (D) 106 magnification of box from (C). Woven bone had replaced the cartilage matrix in every single case.PMID:23667820 (TIF)PLOS 1 | plosone.orgPten Knockouts Have Enhanced Fracture HealingDocument S1 Supplemental approaches.(DOCX)AcknowledgmentsThe authors thank the members on the Mason and Williams labs at VARI for their assistance; David Nadziejka for help preparing the manuscript; DJ Scholten for assisting on surgeries and fractures; Lisa Turner and the VARI histology core for their histology work; Michael Starbuck within the Rolanette and Berdon Lawrence Bone Disease Program of Texas bone histomorphom-etry lab for his support with all the histomorphometry evaluation; VARI’s vivarium employees for outstanding animal husbandry; and Luci Korpi plus the Grand Rapids Region Pre-College Engineering Program for helping with this research.Author ContributionsConceived and developed the experiments: TAB DLS JJM CBJ BOW. Performed the experiments: TAB JZ MFH MRM MAA. Analyzed the data: TAB MFH CJC JJM HLP BOW. Contributed reagents/materials/ evaluation tools: JJM BOW. Wrote the paper: TAB MFH JJM BOW.
NIH Public AccessAuthor ManuscriptLeukemia. Author manuscript; available in PMC 2013 November 19.Published in final edited kind as: Leu.
